| Pos | Name | YOB | Club | Time | ||||
| 1 | Bethany Jerram | 95 | WEWS | 50.22 | ||||
| 2 | Evie Edwards | 95 | FANS | 50.54 | ||||
| 3 | Kira Mancktelow | 95 | HARS | 51.14 | ||||
| 4 | Amy Fisher | 95 | ALTS | 51.58 | ||||
| 5 | Elizabeth Neale | 96 | BBFS | 51.67 | ||||
| 6 | Laura Cullum | 95 | ALTS | 52.80 | ||||
| 7 | Hollie Parker | 96 | LSSS | 55.31 | ||||
| 8 | Lucy Strowger | 95 | GOSS | 57.07 | ||||
| 9 | Catherine Dodge | 95 | HARS | 57.33 | ||||
| 10 | Katie Hodgkinson | 95 | LSSS | 57.68 | ||||
| 11 | Nina Absolom | 96 | WCPS | 58.74 | ||||
| 12 | Aimee Ballantyne | 95 | GOSS | 59.04 | ||||
| 13 | Emma Lothian | 96 | LSSS | 59.47 | ||||
| 14 | Rose Faulkner | 95 | HVWS | 1:00.03 | ||||
| 15 | Hannah Dowdell | 96 | FANS | 1:01.74 | ||||
| 16 | Helene Mathiesen | 96 | WCPS | 1:02.77 | ||||
| 17 | Chloe Salmon | 95 | HVWS | 1:05.17 | ||||
| Katie Mottershead | 96 | LSSS | DQ:1 hand touch at turn | |||||
| Daisy Small | 96 | LSSS | DQ:1 hand touch at turn | |||||
| Grace Parker | 96 | LSSS | DQ:1 hand touch/arms not in same horiz plane | |||||
| Chloe Jackson | 95 | HVWS | DQ:Alternating leg kick at start |