| Pos | Name | YOB | Club | Time | ||||
| 1 | Ella Harding | 93 | TEDS | 41.37 | ||||
| 2 | Laura Gove | 92 | HARS | 43.59 | ||||
| 3 | Amy Frank | 92 | WCPS | 44.96 | ||||
| 4 | Rebecca James | 92 | BBFS | 45.14 | ||||
| 5 | Katie-Jane Bickford-Sawkings | 92 | TEDS | 47.19 | ||||
| 6 | Jocasta Wilson | 93 | RURS | 49.90 | ||||
| 7 | Paige Bayliss | 92 | ANDS | 50.15 | ||||
| Harriet Leavers | 92 | RURS | DQ:Underwater arm recovery | |||||
| Gemma Brown | 92 | GOSS | DQ:FalseStart |